Non-viral delivery using cationic solid lipid nanoparticles (SLNs) represents a useful strategy to introduce large DNA and RNA molecules to target cells. A careful selection of components and their amounts is critical to improve transfection efficiency. In this work, a selected and optimized formulation of SLNs was used to efficiently transfect circular DNA and linear RNA molecules into cells. We characterized the main physicochemical characteristics and binding capabilities of these SLNs and show that they deliver DNA and RNA molecules into cells where they display full bioactivity at nontoxic concentrations using fluorescence- and luminescence-based methodologies. Hence, we established a novel and simple SLN formulation as a powerful tool for future therapeutic use.
Fàbregas A1, Prieto-Sánchez S2, Suñé-Pou M3, Boyero-Corral S2, Ticó JR4, García-Montoya E4, Pérez-Lozano P4, Miñarro M4, Suñé-Negre JM4, Hernández-Munain C5, Suñé C6.
KEYWORDS: Binding efficiency; Cationic solid lipid nanoparticles (SLNs); Luciferase; Octadecylamine (PubChem ID: 15793); Plasmid DNA; Poloxamer 188 (PubChem CID: 24751); Stearic acid (PubChem CID: 5281); Transfection; siRNA